产品货号：
GS4451
中文名称：
重组小鼠FGF-8
英文名称：
Recombinant Murine Fibroblast Growth Factor-8
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 28.1kDa,a single non-glycosylated polypeptide chain containing 246 amino acids.
储存条件：冷冻（-20℃）
概述
Murine FGF-8 is a heparin binding growth factor belonging to the FGF family,which plays a central role during prenatal development,postnatal growth and regeneration of a variety of tissues,by promoting cellular proliferation and differentiation.Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion.Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms(FGF-8 a-h).Murine FGF-8a and b share 100%amino acid identity with that in humans,and murine FGF-8e and f share 98%amino acid identity with humans.None of the FGF-8 isoforms exhibited activity to FGFR1b,2b,3b,but FGFR2c,3c and FGFR4 can be activated by several FGF-8 isoforms.FGF-8 plays an important role in the regulation of embryonic development,cell proliferation,cell differentiation and cell migration,and it is required for normal brain,eye,ear and limb development during embryogenesis.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组小鼠FGF-8，FGF-8, Murine，Recombinant Murine Fibroblast Growth Factor-8
储存条件：冷冻（-20℃）
概述
Murine FGF-8 is a heparin binding growth factor belonging to the FGF family,which plays a central role during prenatal development,postnatal growth and regeneration of a variety of tissues,by promoting cellular proliferation and differentiation.Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion.Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms(FGF-8 a-h).Murine FGF-8a and b share 100%amino acid identity with that in humans,and murine FGF-8e and f share 98%amino acid identity with humans.None of the FGF-8 isoforms exhibited activity to FGFR1b,2b,3b,but FGFR2c,3c and FGFR4 can be activated by several FGF-8 isoforms.FGF-8 plays an important role in the regulation of embryonic development,cell proliferation,cell differentiation and cell migration,and it is required for normal brain,eye,ear and limb development during embryogenesis.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0ng/ml,corresponding to a specific activity of>2.0×105 IU/mg in the presence of 10μg/ml of heparin. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4,500mMNaCl. |
| 内毒素 | Less than 1EU/μg of rMuFGF-8 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TR |
相关搜索：重组小鼠FGF-8，FGF-8, Murine，Recombinant Murine Fibroblast Growth Factor-8